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NXF1 knockdown attenuates inflammatory response in vitro. (A) Expression level of NXF1 mRNA was assessed by qPCR. (B) Protein expression level of NXF1 was detected by Western blotting. (C) Cytokine levels were measured using ELISA. * P < 0.05; ** P < 0.01; *** P < 0.001.

Journal: Frontiers in Immunology

Article Title: RBM15 affects mitophagy in intracerebral hemorrhage by promoting E2F1 nuclear translocation via NXF1

doi: 10.3389/fimmu.2026.1892295

Figure Lengend Snippet: NXF1 knockdown attenuates inflammatory response in vitro. (A) Expression level of NXF1 mRNA was assessed by qPCR. (B) Protein expression level of NXF1 was detected by Western blotting. (C) Cytokine levels were measured using ELISA. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: The following ELISA kits were used for detection: mouse NSE ELISA kit (Elabscience, E-EL-M0089), mouse IL-10 ELISA kit (Elabscience, E-EL-M0046c), mouse IL-6 ELISA kit (Elabscience, E-EL-M0044c), mouse TNF-α ELISA kit (Elabscience, E-EL-M3063), mouse TGF-β ELISA kit (RnDSystems, DY1679), mouse IL-1 ELISA kit (Elabscience, E-EL-M0037c).

Techniques: Knockdown, In Vitro, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

NXF1 down-regulation improves the pathological changes in ICH mice. (A) Changes in lesion volume after NXF1 downregulation in ICH mice. (B) Western blotting was used to detect the expression levels of NXF1 in brain tissue. (C) HE staining was used to detect hematomas in ICH mice. Scale bar: 500 μm (40x), 200 μm (100x). (D) Changes in heart rate, blood pressure, blood glucose level, brain water content, and mNSS in ICH mice. (E) TUNEL staining was used to detect cell apoptosis. Scale bar: 100 μm (200x). (F) FJ-C staining was used to detect apoptosis in neuronal cells. Scale bar: 100 μm (200x). (G) ELISA kits were used to detect the content of IL-6, IL-1β, TNF-α, IL-10, TGF-β, and NSE. * P < 0.05; ** P < 0.01; *** P < 0.001.

Journal: Frontiers in Immunology

Article Title: RBM15 affects mitophagy in intracerebral hemorrhage by promoting E2F1 nuclear translocation via NXF1

doi: 10.3389/fimmu.2026.1892295

Figure Lengend Snippet: NXF1 down-regulation improves the pathological changes in ICH mice. (A) Changes in lesion volume after NXF1 downregulation in ICH mice. (B) Western blotting was used to detect the expression levels of NXF1 in brain tissue. (C) HE staining was used to detect hematomas in ICH mice. Scale bar: 500 μm (40x), 200 μm (100x). (D) Changes in heart rate, blood pressure, blood glucose level, brain water content, and mNSS in ICH mice. (E) TUNEL staining was used to detect cell apoptosis. Scale bar: 100 μm (200x). (F) FJ-C staining was used to detect apoptosis in neuronal cells. Scale bar: 100 μm (200x). (G) ELISA kits were used to detect the content of IL-6, IL-1β, TNF-α, IL-10, TGF-β, and NSE. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: The following ELISA kits were used for detection: mouse NSE ELISA kit (Elabscience, E-EL-M0089), mouse IL-10 ELISA kit (Elabscience, E-EL-M0046c), mouse IL-6 ELISA kit (Elabscience, E-EL-M0044c), mouse TNF-α ELISA kit (Elabscience, E-EL-M3063), mouse TGF-β ELISA kit (RnDSystems, DY1679), mouse IL-1 ELISA kit (Elabscience, E-EL-M0037c).

Techniques: Western Blot, Expressing, Tissue, H&E Stain, TUNEL Assay, Staining, Enzyme-linked Immunosorbent Assay

Angiogenesis and collagen deposition in diabetic wound tissues following HPSL@SG hydrogel treatment. (A) Dihydroethidium (DHE) immunofluorescence staining and (B) semi-quantitative analysis of wound tissues from each treatment group on day 7, scale bar = 100 μm. Immunofluorescence staining of (C) MMP-9, IL-6, and IL-10, and (D) CD31, VEGF-A, and collagen I in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. (E-J) Mean relative fluorescence intensity of each indicator in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. All data are shown as mean ± SEM (n = 6).

Journal: Bioactive Materials

Article Title: Glucose/ROS-responsive and redox-gated adaptive hydrogel dressing for accelerating diabetic wound repair via synergistic cGAS/STING pathway inhibition and oxidative stress alleviation

doi: 10.1016/j.bioactmat.2026.03.025

Figure Lengend Snippet: Angiogenesis and collagen deposition in diabetic wound tissues following HPSL@SG hydrogel treatment. (A) Dihydroethidium (DHE) immunofluorescence staining and (B) semi-quantitative analysis of wound tissues from each treatment group on day 7, scale bar = 100 μm. Immunofluorescence staining of (C) MMP-9, IL-6, and IL-10, and (D) CD31, VEGF-A, and collagen I in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. (E-J) Mean relative fluorescence intensity of each indicator in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. All data are shown as mean ± SEM (n = 6).

Article Snippet: IL-6 and IL-10-specific antibodies were purchased from Bosterbio (Wuhan, China).

Techniques: Immunofluorescence, Staining, Fluorescence